Hotspot

ndhF

SSC · 113065-115284 · 2220 bp

Back to Limonium

Pi 0.0126

MarkerSeek score 52.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 68
pi: 0.0126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GACGTCGTAGAGAATTGCGA	GTCAGAGCCGACGAATCAAA	50.0	50.0	57.8	58.3	2829-2835 bp	1.0	78.7
ndhF_p2	AGACGTCGTAGAGAATTGCG	GTCAGAGCCGACGAATCAAA	50.0	50.0	57.8	58.3	2830-2836 bp	1.0	78.6
ndhF_p3	TGGATAAATAGCTGCGCACA	GTCAGAGCCGACGAATCAAA	45.0	50.0	57.7	58.3	2430-2436 bp	1.0	77.5
ndhF_p4	GACGTCGTAGAGAATTGCGA	TCAGAGCCGACGAATCAAAA	50.0	45.0	57.8	57.6	2828-2834 bp	1.0	77.4
ndhF_p5	AGACGTCGTAGAGAATTGCG	TCAGAGCCGACGAATCAAAA	50.0	45.0	57.8	57.6	2829-2835 bp	1.0	77.4