Hotspot

ndhF-rpl32

SSC · 115285-116208 · 924 bp

Back to Limonium

Pi 0.0275

MarkerSeek score 68.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 924
variable_sites: 60
pi: 0.0275
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCCACATACGCCGAAGTTTA	AATCCCCATCCCAGAACAGA	50.0	50.0	58.0	58.0	1454-1479 bp	1.0	85.9
ndhF-rpl32_p2	TAGGAAAAGCCCACATACGC	AATCCCCATCCCAGAACAGA	50.0	50.0	58.0	58.0	1463-1488 bp	1.0	85.8
ndhF-rpl32_p3	CCCACATACGCCGAAGTTTA	AAGGGTGGCTAAATTGGCAA	50.0	45.0	58.0	58.0	1428 bp	0.2	47.7
ndhF-rpl32_p4	CCCACATACGCCGAAGTTTA	AGGGTGGCTAAATTGGCAAA	50.0	45.0	58.0	58.0	1427 bp	0.2	47.7
ndhF-rpl32_p5	TAGGAAAAGCCCACATACGC	AGGGTGGCTAAATTGGCAAA	50.0	45.0	58.0	58.0	1436 bp	0.2	47.6