Hotspot

petA-psbJ

LSC · 63331-64172 · 842 bp

Back to Limonium

Pi 0.0204
MarkerSeek score 65.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
842
variable_sites
39
pi
0.0204
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TCGAGAAGGTTCAATTGGCT TGGTTCATATTCTGGATTGGGT 45.0 40.9 57.1 57.1 910-928 bp 1.0 55.7
petA-psbJ_p2 CGAGAAGGTTCAATTGGCTG TGGTTCATATTCTGGATTGGGT 50.0 40.9 57.1 57.1 909-927 bp 1.0 55.3
petA-psbJ_p3 TCGAGAAGGTTCAATTGGCT ATTCTGGATTGGGTTCATCC 45.0 45.0 57.1 55.1 902-920 bp 1.0 55.2
petA-psbJ_p4 CGAGAAGGTTCAATTGGCTG ATTCTGGATTGGGTTCATCC 50.0 45.0 57.1 55.1 901-919 bp 1.0 54.8
petA-psbJ_p5 TTCGAGAAGGTTCAATTGGCT TGGTTCATATTCTGGATTGGGT 42.9 40.9 57.9 57.1 911-929 bp 1.0 53.4