Hotspot

ycf4-cemA

LSC · 60939-61472 · 534 bp

Back to Limonium

Pi 0.0202

MarkerSeek score 55.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 27
pi: 0.0202
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGAGAATTTGACTCCGCGAG	CGGGAAGGGTAGAAACAAGG	50.0	55.0	58.0	57.9	1207-1222 bp	1.0	82.9
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	GGAAGGGTAGAAACAAGGCC	50.0	55.0	58.0	58.2	1205-1220 bp	1.0	82.5
ycf4-cemA_p3	TGAGAATTTGACTCCGCGAG	GGGAAGGGTAGAAACAAGGC	50.0	55.0	58.0	58.2	1206-1221 bp	1.0	82.5
ycf4-cemA_p4	TGAGAATTTGACTCCGCGAG	GCTACAGAAATCCACCAGGG	50.0	55.0	58.0	58.0	689-704 bp	0.8	72.5
ycf4-cemA_p5	ACCCCCGAAAGTTCACATTC	GCTACAGAAATCCACCAGGG	50.0	55.0	58.1	58.0	1336-1351 bp	0.8	71.5