Hotspot

rbcL-accD

LSC · 57481-58154 · 674 bp

Back to Limonium

Pi 0.016

MarkerSeek score 60.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 27
pi: 0.016
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATTCGCCGCAATGGATACT	CCTGCGTAACCAACATCGAA	45.0	50.0	58.0	58.6	739-766 bp	1.0	78.7
rbcL-accD_p2	ATTCGCCGCAATGGATACTT	CCTGCGTAACCAACATCGAA	45.0	50.0	58.0	58.6	738-765 bp	1.0	78.7
rbcL-accD_p3	AATTCGCCGCAATGGATACT	TTCCTGCGTAACCAACATCG	45.0	50.0	58.0	58.6	741-768 bp	1.0	78.6
rbcL-accD_p4	ATTCGCCGCAATGGATACTT	TTCCTGCGTAACCAACATCG	45.0	50.0	58.0	58.6	740-767 bp	1.0	78.6
rbcL-accD_p5	TGAATTCGCCGCAATGGATA	CCTGCGTAACCAACATCGAA	45.0	50.0	58.0	58.6	741-768 bp	1.0	78.6