Hotspot

petN-psbM

LSC · 28542-29602 · 1061 bp

Back to Limonium

Pi 0.0115

MarkerSeek score 62.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 30
pi: 0.0115
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AAAACGGTCAGCCAAAATGA	45.0	40.0	58.0	56.4	1158-1161 bp	1.0	70.2
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	AAAACGGTCAGCCAAAATGA	50.0	40.0	57.8	56.4	1164-1167 bp	1.0	68.7
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	AAACGGTCAGCCAAAATGAT	45.0	40.0	58.0	56.2	1157-1160 bp	1.0	68.3
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	AACGGTCAGCCAAAATGATT	45.0	40.0	58.0	56.2	1156-1159 bp	1.0	68.3
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	AAAAACGGTCAGCCAAAATGA	45.0	38.1	58.0	57.2	1159-1162 bp	1.0	68.2