Hotspot

rpoB-trnC-GCA

LSC · 26160-27505 · 1346 bp

Back to Limonium

Pi 0.0167

MarkerSeek score 68.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 56
pi: 0.0167
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1423-1468 bp	1.0	85.2
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	CCAGATTTGAACCGGGGAAA	50.0	50.0	58.0	58.1	1464-1509 bp	1.0	84.9
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATCAATGA	GGGATTTCGGGTCTGTTGTT	50.0	50.0	58.0	58.1	1497-1542 bp	1.0	84.7
rpoB-trnC-GCA_p4	CGTCAAGCCCTGATCAATGA	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.0	58.2	1444-1489 bp	1.0	84.3
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATCAATGA	TCTGTTGTTGATCAGGCGAC	50.0	50.0	58.0	58.2	1486-1531 bp	1.0	83.9