Hotspot

trnG-UCC-trnR-UCU

LSC · 9154-9308 · 155 bp

Back to Limonium

Pi 0.0361

MarkerSeek score 59.50

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 14
pi: 0.0361
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	GCATATGGAAATAAATTGCGTCC	50.0	39.1	58.0	56.9	287-296 bp	1.0	51.0
trnG-UCC-trnR-UCU_p2	ACCCTTAGCCTTCCAAGCTA	GCATATGGAAATAAATTGCGTCC	50.0	39.1	58.0	56.9	293-302 bp	1.0	50.9
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAATAGG	50.0	37.5	58.0	57.9	282-291 bp	1.0	50.6
trnG-UCC-trnR-UCU_p4	ACCCTTAGCCTTCCAAGCTA	TGGAAATAAATTGCGTCCAATAGG	50.0	37.5	58.0	57.9	288-297 bp	1.0	50.5
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	AGCATATGGAAATAAATTGCGTCC	50.0	37.5	58.0	58.2	288-297 bp	1.0	49.8