Hotspot

trnS-GCU-trnG-UCC

LSC · 7590-8388 · 799 bp

Back to Limonium

Pi 0.0168

MarkerSeek score 62.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 33
pi: 0.0168
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCCTCGGTACAAATCACTCG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	899-933 bp	1.0	54.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	920-954 bp	1.0	53.9
trnS-GCU-trnG-UCC_p3	CCCTCGGTACAAATCACTCG	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.1	57.3	902-936 bp	1.0	53.5
trnS-GCU-trnG-UCC_p4	CCCTCGGTACAAATCACTCG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.1	57.3	901-935 bp	1.0	53.5
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	922-956 bp	1.0	52.8