Hotspot

rps16-trnQ-UUG

LSC · 5942-6288 · 347 bp

Back to Limonium

Pi 0.029

MarkerSeek score 71.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 20
pi: 0.029
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCCACACCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	408-442 bp	1.0	86.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	386-420 bp	1.0	86.2
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	397-431 bp	1.0	85.6
rps16-trnQ-UUG_p4	TCCACACCAGATCATGTCCT	ATATTTTAGTGGGGCGTGGC	50.0	50.0	58.0	58.3	465-499 bp	1.0	84.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	ATATTTTAGTGGGGCGTGGC	45.0	50.0	58.1	58.3	443-477 bp	1.0	83.8