Hotspot

ycf1

SSC · 107654-113065 · 5412 bp

Back to Limnophila

Pi 0.0367

MarkerSeek score 61.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5412
variable_sites: 197
pi: 0.0367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTATGCGAGCTCAAAGGC	TCTTCTTCTGTATACTCACCAAC	50.0	39.1	58.3	55.5	195 bp	1.0	48.6
ycf1_p2	TTCTATGCGAGCTCAAAGGC	TGTTCTTCTTCTGTATACTCACCA	50.0	37.5	58.3	57.1	198 bp	0.5	20.0
ycf1_p3	TTCTATGCGAGCTCAAAGGC	TGTTCTTCTTCTGTATACTCACCAA	50.0	36.0	58.3	57.7	198 bp	0.5	20.0
ycf1_p4	TTCTATGCGAGCTCAAAGGC	TTGTTCTTCTTCTGTATACTCACCA	50.0	36.0	58.3	57.7	199 bp	0.5	20.0
ycf1_p5	TTCTATGCGAGCTCAAAGGC	GTTCTTCTTCTGTATACTCACCA	50.0	39.1	58.3	55.5	197 bp	0.5	20.0