Hotspot

trnL-UAG-rpl32

SSC · 122802-123590 · 789 bp

Back to Limnophila

Pi 0.0474

MarkerSeek score 74.80

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 789
variable_sites: 37
pi: 0.0474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AAAAGGCCGGAAGTAGCAAA	ACTTCTGCATCAAAAACGCG	45.0	45.0	58.0	58.0	1511-1612 bp	1.0	83.8
trnL-UAG-rpl32_p2	AAAAGGCCGGAAGTAGCAAA	CTTCTGCATCAAAAACGCGT	45.0	45.0	58.0	58.0	1510-1611 bp	1.0	83.8
trnL-UAG-rpl32_p3	AGCCTTGGGTAAAAATGGCA	ACTTCTGCATCAAAAACGCG	45.0	45.0	58.0	58.0	1492-1593 bp	1.0	83.7
trnL-UAG-rpl32_p4	AGCCTTGGGTAAAAATGGCA	CTTCTGCATCAAAAACGCGT	45.0	45.0	58.0	58.0	1491-1592 bp	1.0	83.7
trnL-UAG-rpl32_p5	AAAAGGCCGGAAGTAGCAAA	AAAAAGCGCACTTCTGCATC	45.0	45.0	58.0	57.9	1520-1621 bp	1.0	83.7