Hotspot

psaC-ndhD

SSC · 119793-119913 · 121 bp

Back to Limnophila

Pi 0.0992

MarkerSeek score 73.70

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 121
variable_sites: 12
pi: 0.0992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	GCTATTGGCCTGGCTATTGT	CCTATTTGCCCGCTGTACAT	50.0	50.0	58.0	58.0	1095-1110 bp	1.0	83.8
psaC-ndhD_p2	GATACCCTGGGACGGATGTA	CCTATTTGCCCGCTGTACAT	55.0	50.0	58.0	58.0	664 bp	1.0	82.5
psaC-ndhD_p3	TCTGCTCCAAGAACTGAGGA	CCTATTTGCCCGCTGTACAT	50.0	50.0	58.0	58.0	627 bp	0.5	59.6
psaC-ndhD_p4	CTGCTCCAAGAACTGAGGAC	CCTATTTGCCCGCTGTACAT	55.0	50.0	57.9	58.0	626 bp	0.5	59.0
psaC-ndhD_p5	GCTCCAAGAACTGAGGACTG	CCTATTTGCCCGCTGTACAT	55.0	50.0	57.9	58.0	624 bp	0.5	59.0