Hotspot

ycf1-rps15

SSC · 113066-113509 · 444 bp

Back to Limnophila

Pi 0.0498

MarkerSeek score 74.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 444
variable_sites: 21
pi: 0.0498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGATCATAGAGCCCCTTCGT	CCAAATAGGTTAGCAGCCGT	50.0	50.0	57.9	58.0	1164-1176 bp	1.0	83.7
ycf1-rps15_p2	TGATCATAGAGCCCCTTCGT	TCCCAACGCGTTACATAAGG	50.0	50.0	57.9	58.0	1544-1556 bp	1.0	83.1
ycf1-rps15_p3	TGATCATAGAGCCCCTTCGT	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	1407-1419 bp	1.0	83.0
ycf1-rps15_p4	TGATCATAGAGCCCCTTCGT	TGCATTGATGGGTGATGAGG	50.0	50.0	57.9	57.9	1400-1412 bp	1.0	83.0
ycf1-rps15_p5	TGATCATAGAGCCCCTTCGT	GCATTGATGGGTGATGAGGT	50.0	50.0	57.9	57.9	1399-1411 bp	1.0	82.9