Hotspot

trnW-CCA-trnP-UGG

LSC · 66025-66188 · 164 bp

Back to Limnophila

Pi 0.0671

MarkerSeek score 73.90

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 11
pi: 0.0671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	233-234 bp	1.0	82.6
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	231-232 bp	1.0	82.6
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	234-235 bp	1.0	82.6
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	244-245 bp	1.0	82.5
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	245-246 bp	1.0	82.5