Hotspot

petG-trnW-CCA

LSC · 65822-65950 · 129 bp

Back to Limnophila

Pi 0.062

MarkerSeek score 73.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 8
pi: 0.062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CAATACAGGCGTGGTGATCA	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.0	58.0	238 bp	1.0	82.5
petG-trnW-CCA_p2	ACAATACAGGCGTGGTGATC	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.0	58.0	239 bp	1.0	82.5
petG-trnW-CCA_p3	CAATACAGGCGTGGTGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	215 bp	1.0	82.5
petG-trnW-CCA_p4	ACAATACAGGCGTGGTGATC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	216 bp	1.0	82.5
petG-trnW-CCA_p5	CAATACAGGCGTGGTGATCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	201 bp	1.0	82.3