Hotspot

psbE-petL

LSC · 64481-65433 · 953 bp

Back to Limnophila

Pi 0.026

MarkerSeek score 71.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 24
pi: 0.026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1129-1158 bp	1.0	75.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1039-1068 bp	1.0	75.2
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1034-1063 bp	1.0	74.9
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1125-1154 bp	1.0	74.8
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	1032-1061 bp	1.0	73.7