Hotspot

trnE-UUC-trnT-GGU

LSC · 30789-31366 · 578 bp

Back to Limnophila

Pi 0.0702
MarkerSeek score 82.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
578
variable_sites
40
pi
0.0702
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 681-715 bp 1.0 83.3
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 660-694 bp 1.0 83.1
trnE-UUC-trnT-GGU_p3 ACTTCCTTTCGTGGTACCCT GAACCGATGACTTACGCCTT 50.0 50.0 58.3 58.0 725-759 bp 1.0 82.9
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 657-691 bp 1.0 82.9
trnE-UUC-trnT-GGU_p5 ACTTCCTTTCGTGGTACCCT CCATGGCGTTACTCTACCAC 50.0 55.0 58.3 58.1 704-738 bp 1.0 82.6