Hotspot

rpoB-trnC-GCA

LSC · 26789-28270 · 1482 bp

Back to Limnophila

Pi 0.0466

MarkerSeek score 75.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1482
variable_sites: 64
pi: 0.0466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	2001-2056 bp	1.0	85.8
rpoB-trnC-GCA_p2	CCGCCTACACAAGCAAATTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	2193-2248 bp	1.0	85.5
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TCGTACCCCCTTCCAGTATC	50.0	55.0	58.0	57.9	2369-2402 bp	1.0	85.5
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	TCGTACCCCCTTCCAGTATC	50.0	55.0	58.0	57.9	2561-2594 bp	1.0	85.1
rpoB-trnC-GCA_p5	ATATTGGATCCCCGCCTACA	TCGTACCCCCTTCCAGTATC	50.0	55.0	58.0	57.9	2572-2605 bp	1.0	84.9