Hotspot

atpH-atpI

LSC · 13401-14423 · 1023 bp

Back to Limnophila

Pi 0.0416

MarkerSeek score 75.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 42
pi: 0.0416
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TCCCTGTGATGTTCCTTGGA	45.0	50.0	58.1	58.3	1151-1157 bp	1.0	81.0
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	CGCGGCTTATATCGGTGAAT	45.0	50.0	58.1	58.3	1087-1093 bp	1.0	81.0
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TCCCTGTGATGTTCCTTGGA	50.0	50.0	57.8	58.3	1168-1174 bp	1.0	80.0
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	CGCGGCTTATATCGGTGAAT	50.0	50.0	57.8	58.3	1104-1110 bp	1.0	80.0
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	TCCCTGTGATGTTCCTTGGA	50.0	50.0	58.3	58.3	1163-1169 bp	1.0	79.7