Hotspot

rps16-trnQ-UUG

LSC · 5684-6519 · 836 bp

Back to Limnophila

Pi 0.0309

MarkerSeek score 71.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 25
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	911-926 bp	1.0	83.4
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	GAACAAACTGAAATGGGGCG	50.0	50.0	57.9	57.9	972-987 bp	1.0	83.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	894-909 bp	1.0	83.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAACAAACTGAAATGGGGCG	45.0	50.0	58.1	57.9	955-970 bp	1.0	82.8
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GAACAAACTGAAATGGGGCG	50.0	50.0	58.2	57.9	973-988 bp	1.0	82.1