Hotspot

rps19

LSC · 81860-82138 · 279 bp

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Pi 0.00656
MarkerSeek score 32.70
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
16
pi
0.00656
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 CATCTTCCTGTATACCCGCG GTTATCCTGCGCTTGGAAGA 55.0 50.0 57.9 58.0 1230-1326 bp 0.969697 89.2
rps19_p2 CATCTTCCTGTATACCCGCG TGGGTAGAGCCGGATCTAAG 55.0 55.0 57.9 58.0 1368-1464 bp 0.969697 88.8
rps19_p3 TTTCTGTACTCCAACGCGAG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 711-805 bp 0.878788 85.2
rps19_p4 TTTCTGTACTCCAACGCGAG TGGGTAGAGCCGGATCTAAG 50.0 55.0 57.9 58.0 849-943 bp 0.878788 84.5
rps19_p5 TTTCTGTACTCCAACGCGAG GAGGGTAAATCGGCCACATT 50.0 50.0 57.9 57.9 961-1055 bp 0.878788 83.7