Hotspot

rpoA

LSC · 75700-76707 · 1008 bp

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Pi 0.00681
MarkerSeek score 46.20
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1008
variable_sites
60
pi
0.00681
unique_haplotype_count
37
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 GCCCAGTAGCTACAACAGTC CGAGTAGTTTCTTGGGCCTC 55.0 55.0 58.0 58.0 1665-1704 bp 0.984848 86.1
rpoA_p2 GCCCAGTAGCTACAACAGTC CACGCAAGAATGGACGTAGA 55.0 50.0 58.0 58.0 1757-1796 bp 0.984848 85.8
rpoA_p3 GCCCAGTAGCTACAACAGTC TCACGCAAGAATGGACGTAG 55.0 50.0 58.0 58.0 1758-1797 bp 0.984848 85.8
rpoA_p4 GGATCCATTTGCGACTCCTT TGCTATTCGTACGGTGGTTG 50.0 50.0 58.0 58.0 1760-1794 bp 0.878788 80.0
rpoA_p5 GCCCAGTAGCTACAACAGTC TGCTATTCGTACGGTGGTTG 55.0 50.0 58.0 58.0 1581-1615 bp 0.863636 80.0