Hotspot

rpl32-trnL-UAG

SSC · 111304-112014 · 711 bp

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Pi 0.0205
MarkerSeek score 60.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
711
variable_sites
102
pi
0.0205
unique_haplotype_count
48
primer_available
yes
alignment_reliability
0.88
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TAAATCGGTTTCCACTGGGC ACTGCTTCCTAAGAACAGCG 50.0 50.0 58.2 57.9 624-867 bp 0.893939 85.7
rpl32-trnL-UAG_p2 TAAATCGGTTTCCACTGGGC CTGCTTCCTAAGAACAGCGT 50.0 50.0 58.2 57.9 623-866 bp 0.893939 85.7
rpl32-trnL-UAG_p3 TAAATCGGTTTCCACTGGGC GCTTCCTAAGAACAGCGTGT 50.0 50.0 58.2 58.2 621-864 bp 0.893939 84.8
rpl32-trnL-UAG_p4 TAAATCGGTTTCCACTGGGC ACTGAGATTGTAGAGCGGGT 50.0 50.0 58.2 58.1 1144-1387 bp 0.893939 84.6
rpl32-trnL-UAG_p5 TAAATCGGTTTCCACTGGGC TGGCGAAACCTTGGGTAAAA 50.0 45.0 58.2 57.9 1081-1324 bp 0.878788 84.1