Hotspot

ndhF-rpl32

SSC · 110723-111129 · 407 bp

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Pi 0.0167
MarkerSeek score 62.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
407
variable_sites
46
pi
0.0167
unique_haplotype_count
52
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGAAAGGGTATTATCCATGCGT AGAAAGAGCTTTTACTGCCGT 40.9 42.9 57.3 57.9 540-559 bp 0.969697 57.6
ndhF-rpl32_p2 AAGAAAGGGTATTATCCATGCGT AGAAAGAGCTTTTACTGCCGT 39.1 42.9 58.0 57.9 541-560 bp 0.969697 54.9
ndhF-rpl32_p3 GAAAGGGTATTATCCATGCGT AGAAAGAGCTTTTACTGCCGT 42.9 42.9 55.8 57.9 539-558 bp 0.969697 53.8
ndhF-rpl32_p4 AGAAAGGGTATTATCCATGCGT GAAAGAGCTTTTACTGCCGT 40.9 45.0 57.3 56.4 539-558 bp 0.969697 53.6
ndhF-rpl32_p5 AGAAAGGGTATTATCCATGCGT GAAAGAGCTTTTACTGCCGTT 40.9 42.9 57.3 57.1 539-552 bp 0.954545 50.3