Hotspot

trnF-GAA-ndhJ

LSC · 45946-46715 · 770 bp

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Pi 0.0136
MarkerSeek score 62.10
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
770
variable_sites
72
pi
0.0136
unique_haplotype_count
42
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAATACAAGATGCTCATTGA 50.0 31.8 58.1 53.8 779-834 bp 1.0 51.1
trnF-GAA-ndhJ_p2 AAAGGTCGGGATAGCTCAGT TGAAATACAAGATGCTCATTGA 50.0 31.8 58.1 53.8 822-877 bp 1.0 50.7
trnF-GAA-ndhJ_p3 AAGGTCGGGATAGCTCAGTT TGAAATACAAGATGCTCATTGA 50.0 31.8 58.1 53.8 821-876 bp 1.0 50.7
trnF-GAA-ndhJ_p4 CGAGAAAAGGTCGGGATAGC TGAAATACAAGATGCTCATTGA 55.0 31.8 58.1 53.8 827-882 bp 1.0 50.7
trnF-GAA-ndhJ_p5 GCGAGAAAAGGTCGGGATAG TGAAATACAAGATGCTCATTGA 55.0 31.8 58.1 53.8 828-883 bp 1.0 50.7