Hotspot

ycf3-trnS-GGA

LSC · 42362-42933 · 572 bp

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Pi 0.0113
MarkerSeek score 61.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
572
variable_sites
46
pi
0.0113
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTTCCCCTGAAGTTGTCGG ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.0 58.2 711-760 bp 1.0 87.9
ycf3-trnS-GGA_p2 TTTTCCCCTGAAGTTGTCGG TTCCAATGCTACGCCTTGAA 50.0 45.0 58.0 57.8 661-710 bp 1.0 87.8
ycf3-trnS-GGA_p3 TTTTCCCCTGAAGTTGTCGG CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.0 58.1 690-739 bp 0.984848 87.8
ycf3-trnS-GGA_p4 CCCCTGAAGTTGTCGGAATT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 707-756 bp 1.0 86.5
ycf3-trnS-GGA_p5 CCCCTGAAGTTGTCGGAATT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.8 58.1 686-735 bp 0.984848 86.4