Hotspot

psbZ-trnG-GCC

LSC · 34304-34590 · 287 bp

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Pi 0.0199
MarkerSeek score 65.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
287
variable_sites
30
pi
0.0199
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTTCTGACGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 58.4 58.1 447-482 bp 1.0 86.1
psbZ-trnG-GCC_p2 TTGCTTCTTCTGACGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 58.4 58.1 448-483 bp 1.0 86.1
psbZ-trnG-GCC_p3 GCTTCTTCTGACGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.9 58.1 445-480 bp 1.0 82.6
psbZ-trnG-GCC_p4 GCTTCTTCTGACGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.9 58.1 446-481 bp 1.0 82.6
psbZ-trnG-GCC_p5 TTGCTTCTTCTGACGGTTGG GAACCCGCATCTTCTCCTTG 50.0 55.0 58.4 58.6 443-478 bp 1.0 81.6