Hotspot

trnT-GGU-psbD

LSC · 30364-31265 · 902 bp

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Pi 0.0143
MarkerSeek score 61.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
902
variable_sites
82
pi
0.0143
unique_haplotype_count
51
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CACCGTCACCATCTTCGAAT 55.0 50.0 58.1 58.0 1604-1825 bp 1.0 88.1
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG TGAACAGATCGAGCAAGCTC 55.0 50.0 58.1 58.0 1329-1550 bp 0.954545 86.7
trnT-GGU-psbD_p3 GGTAGTGCTTGATCCGACAG TGAACAGATCGAGCAAGCTC 55.0 50.0 58.1 58.0 1723-1961 bp 0.939394 86.4
trnT-GGU-psbD_p4 TAATGGAATTTGGCTCGGGG CACCGTCACCATCTTCGAAT 50.0 50.0 57.9 58.0 1802-2021 bp 0.712121 76.1
trnT-GGU-psbD_p5 TAATGGAATTTGGCTCGGGG TGAACAGATCGAGCAAGCTC 50.0 50.0 57.9 58.0 1527-1746 bp 0.666667 74.5