Hotspot

psbM-trnD-GUC

LSC · 27874-29055 · 1182 bp

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Pi 0.00969
MarkerSeek score 63.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1182
variable_sites
99
pi
0.00969
unique_haplotype_count
54
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTGACTGGCTGTTTTTACGT CCTCGCTGGGATTGTAGTTC 40.0 55.0 55.8 58.1 768-1373 bp 1.0 72.7
psbM-trnD-GUC_p2 TTGACTGGCTGTTTTTACGT AGTTCAATCGGTCAGAGCAC 40.0 50.0 55.8 57.9 753-1358 bp 1.0 72.4
psbM-trnD-GUC_p3 GCGGTAGGAACTAGAATGAACA CCTCGCTGGGATTGTAGTTC 45.5 55.0 57.9 58.1 731-1336 bp 0.969697 72.1
psbM-trnD-GUC_p4 GCGGTAGGAACTAGAATGAACA AGTTCAATCGGTCAGAGCAC 45.5 50.0 57.9 57.9 716-1321 bp 0.969697 71.8
psbM-trnD-GUC_p5 GCGGTAGGAACTAGAATGAACA TACACCTCGCTGGGATTGTA 45.5 50.0 57.9 58.1 735-1340 bp 0.969697 71.3