Hotspot

trnS-GCU-trnG-UCC

LSC · 7591-8270 · 680 bp

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Pi 0.0201
MarkerSeek score 66.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
680
variable_sites
76
pi
0.0201
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.95
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA TGTCGGAAACTGAAAGACCC 45.0 50.0 58.0 57.8 723-840 bp 1.0 89.6
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA CGGAAACTGAAAGACCCCTT 45.0 50.0 58.0 57.7 720-837 bp 1.0 89.5
trnS-GCU-trnG-UCC_p3 GAGGGATAGCGGAGAGAGAG TGTCGGAAACTGAAAGACCC 60.0 50.0 58.2 57.8 782-899 bp 1.0 88.2
trnS-GCU-trnG-UCC_p4 GAGGGATAGCGGAGAGAGAG CGGAAACTGAAAGACCCCTT 60.0 50.0 58.2 57.7 779-896 bp 1.0 88.1
trnS-GCU-trnG-UCC_p5 GATGAGGGATAGCGGAGAGA TGTCGGAAACTGAAAGACCC 55.0 50.0 57.8 57.8 785-902 bp 1.0 88.0