Hotspot

rps16-trnQ-UUG

LSC · 5959-6583 · 625 bp

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Pi 0.0174
MarkerSeek score 66.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
625
variable_sites
66
pi
0.0174
unique_haplotype_count
48
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 364-865 bp 1.0 89.1
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 375-876 bp 1.0 88.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 368-869 bp 1.0 88.0
rps16-trnQ-UUG_p4 GGCGGATGTAAGAATCCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 400-901 bp 1.0 87.5
rps16-trnQ-UUG_p5 TGGCGGATGTAAGAATCCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 401-902 bp 1.0 87.5