Pi
0.025
MarkerSeek score
59.70
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | TGAACGGACCATATCGGAGA | GACAATTTGGAATGCTGGCC | 50.0 | 50.0 | 57.9 | 58.0 | 740-1289 bp | 1.0 | 91.4 |
| ycf1_p2 | TGAACGGACCATATCGGAGA | CTTGCCCATATGGAGTGGTC | 50.0 | 55.0 | 57.9 | 58.0 | 879-1533 bp | 1.0 | 91.3 |
| ycf1_p3 | TGAACGGACCATATCGGAGA | ACGCCTTTGAGCACGTATAG | 50.0 | 50.0 | 57.9 | 58.1 | 575-1124 bp | 1.0 | 90.9 |
| ycf1_p4 | TGAACGGACCATATCGGAGA | CGCATGTGCTTGAAACAGTT | 50.0 | 45.0 | 57.9 | 57.9 | 611-1160 bp | 1.0 | 90.5 |
| ycf1_p5 | TGAACGGACCATATCGGAGA | TGCTATCCCGATTCTCTCGT | 50.0 | 50.0 | 57.9 | 58.0 | 974-1502 bp | 0.307692 | 57.0 |
| ycf1_p1 | GACAATTTGGAATGCTGGCC | CGCTCAAGAAACGCCAAATT | 50.0 | 45.0 | 58.0 | 57.9 | 333-807 bp | 1.0 | 91.5 |
| ycf1_p2 | GACAATTTGGAATGCTGGCC | TGAACGGACCATATCGGAGA | 50.0 | 50.0 | 58.0 | 57.9 | 740-1289 bp | 1.0 | 91.4 |
| ycf1_p3 | CTTGCCCATATGGAGTGGTC | CGCTCAAGAAACGCCAAATT | 55.0 | 45.0 | 58.0 | 57.9 | 451-1051 bp | 1.0 | 91.4 |
| ycf1_p4 | TGCTATCCCGATTCTCTCGT | CGCTCAAGAAACGCCAAATT | 50.0 | 45.0 | 58.0 | 57.9 | 552-1020 bp | 0.307692 | 57.0 |
| ycf1_p5 | TGCTATCCCGATTCTCTCGT | TGAACGGACCATATCGGAGA | 50.0 | 50.0 | 58.0 | 57.9 | 974-1502 bp | 0.307692 | 57.0 |