Hotspot

atpB-rbcL

LSC · 56356-57153 · 798 bp

Back to Ligustrum

Pi 0.0177

MarkerSeek score 57.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 36
pi: 0.0177
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	809-900 bp	1.0	84.3
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	55.0	45.0	58.1	57.4	810-901 bp	1.0	82.1
atpB-rbcL_p3	ACCCCAGAACCAGAAGTAGT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.6	58.3	805-896 bp	1.0	81.6
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	CCCGCTTTGAATCCAACACT	55.0	50.0	58.1	58.8	805-896 bp	1.0	80.7
atpB-rbcL_p5	TTTTTCAAGCGTGGAAACCC	AACACCCGCTTTGAATCCAA	45.0	45.0	57.4	58.3	821-912 bp	1.0	80.2