Hotspot

psaA-pafI

LSC · 43403-44110 · 708 bp

Back to Ligustrum

Pi 0.0107

MarkerSeek score 60.80

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 24
pi: 0.0107
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	861-874 bp	1.0	82.7
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	50.0	45.0	57.9	57.7	886-899 bp	1.0	80.7
psaA-pafI_p3	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	50.0	50.0	57.9	57.7	855-868 bp	1.0	80.7
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	TTGGAAACAGGCTATAGCGC	50.0	50.0	57.9	58.3	863-876 bp	1.0	80.3
psaA-pafI_p5	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	853-866 bp	1.0	80.2