Hotspot

trnH-GUG-psbA

LSC · 89-517 · 429 bp

Back to Ligustrum

Pi 0.0178

MarkerSeek score 57.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 26
pi: 0.0178
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	543-555 bp	1.0	82.0
trnH-GUG-psbA_p2	TTAGTTATGGGCGAACGACG	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.1	58.3	598-610 bp	1.0	81.9
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	544-556 bp	1.0	81.8
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	544-556 bp	1.0	81.6
trnH-GUG-psbA_p5	TTAGTTATGGGCGAACGACG	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.1	57.6	599-611 bp	1.0	81.4