primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	89	517	543-555	1.000000	82.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TTAGTTATGGGCGAACGACG	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.424304	89	517	598-610	1.000000	81.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	58.2	58.3	0.0	0.0	0.496882	89	517	544-556	1.000000	81.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531379	89	517	544-556	1.000000	81.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TTAGTTATGGGCGAACGACG	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.484588	89	517	599-611	1.000000	81.4
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.938078	9173	9302	221-227	0.923077	74.1
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.939466	9173	9302	222-228	0.923077	74.0
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.042943	9173	9302	240-246	0.923077	72.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.230929	9173	9302	221-227	0.923077	71.7
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.232316	9173	9302	222-228	0.923077	71.7
rps2-rpoC2_p1	rps2-rpoC2	1	GCCAAAATGAACTCCTGCCT	TCCATGATACATCAGAACAATCA	20	23	50.0	34.8	58.7	55.5	0.0	0.0	6.276179	17363	17561	304-321	1.000000	47.4
rps2-rpoC2_p2	rps2-rpoC2	2	CCATGGCCAAAATGAACTCC	TCCATGATACATCAGAACAATCA	20	23	50.0	34.8	57.3	55.5	11.0	0.0	6.215194	17363	17561	309-326	1.000000	47.3
rps2-rpoC2_p3	rps2-rpoC2	3	TTGCCGAGATATAAGGTGCC	TCCATGATACATCAGAACAATCA	20	23	50.0	34.8	57.5	55.5	0.0	0.0	6.080500	17363	17561	353-370	1.000000	46.7
rps2-rpoC2_p4	rps2-rpoC2	4	TGCCGAGATATAAGGTGCCA	TCCATGATACATCAGAACAATCA	20	23	50.0	34.8	58.6	55.5	0.0	0.0	6.117342	17363	17561	352-369	1.000000	46.7
rps2-rpoC2_p5	rps2-rpoC2	5	GCCGAGATATAAGGTGCCAT	TCCATGATACATCAGAACAATCA	20	23	50.0	34.8	57.2	55.5	0.0	0.0	6.306887	17363	17561	351-368	1.000000	46.7
psaA-pafI_p1	psaA-pafI	1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	43403	44110	861-874	1.000000	82.7
psaA-pafI_p2	psaA-pafI	2	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	20	20	50.0	45.0	57.9	57.7	4.1	20.4	0.390477	43403	44110	886-899	1.000000	80.7
psaA-pafI_p3	psaA-pafI	3	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	20	20	50.0	50.0	57.9	57.7	4.1	18.7	0.411415	43403	44110	855-868	1.000000	80.7
psaA-pafI_p4	psaA-pafI	4	GCCCATTCCTCGAAAGAAGT	TTGGAAACAGGCTATAGCGC	20	20	50.0	50.0	57.9	58.3	4.1	18.3	0.451648	43403	44110	863-876	1.000000	80.3
psaA-pafI_p5	psaA-pafI	5	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	20	20	50.0	55.0	57.9	57.6	4.1	18.7	0.471060	43403	44110	853-866	1.000000	80.2
atpB-rbcL_p1	atpB-rbcL	1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	56356	57153	809-900	1.000000	84.3
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.659357	56356	57153	810-901	1.000000	82.1
atpB-rbcL_p3	atpB-rbcL	3	ACCCCAGAACCAGAAGTAGT	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.6	58.3	0.0	0.0	0.719213	56356	57153	805-896	1.000000	81.6
atpB-rbcL_p4	atpB-rbcL	4	GGAAACCCCAGAACCAGAAG	CCCGCTTTGAATCCAACACT	20	20	55.0	50.0	58.1	58.8	0.0	0.0	0.841376	56356	57153	805-896	1.000000	80.7
atpB-rbcL_p5	atpB-rbcL	5	TTTTTCAAGCGTGGAAACCC	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	57.4	58.3	5.5	0.0	0.889521	56356	57153	821-912	1.000000	80.2
rbcL-trnT-GGU_p1	rbcL-trnT-GGU	1	ACTACAGTTCGGTGGAGGAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.0	58.1	0.0	7.6	0.069438	58588	59530	1239-1239	0.230769	48.7
rbcL-trnT-GGU_p2	rbcL-trnT-GGU	2	GGCTTCAGGGGGTATTCATG	GTGGTAGAGTAACGCCATGG	20	20	55.0	55.0	58.0	58.1	0.0	7.6	0.077185	58588	59530	1305-1305	0.230769	48.6
rbcL-trnT-GGU_p3	rbcL-trnT-GGU	3	TTGAATGCTACTGCGGGTAC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.0	58.1	0.0	7.6	0.082609	58588	59530	1718-1718	0.230769	48.6
rbcL-trnT-GGU_p4	rbcL-trnT-GGU	4	ACTACAGTTCGGTGGAGGAA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.091592	58588	59530	1242-1242	0.230769	48.5
rbcL-trnT-GGU_p5	rbcL-trnT-GGU	5	GGCTTCAGGGGGTATTCATG	TCAGTGGTAGAGTAACGCCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.099340	58588	59530	1308-1308	0.230769	48.4
trnT-GGU-accD_p1	trnT-GGU-accD	1	GAACCGATGACTTACGCCTT	CTTGCAATGACCGCTCCTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046929	59603	62115	2465-2687	0.230769	54.0
trnT-GGU-accD_p2	trnT-GGU-accD	2	GAACCGATGACTTACGCCTT	CTGACGATCACTCTCAAGCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.086052	59603	62115	2496-2718	0.230769	53.6
trnT-GGU-accD_p3	trnT-GGU-accD	3	CCATGGCGTTACTCTACCAC	CTTGCAATGACCGCTCCTAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.105026	59603	62115	2444-2666	0.230769	53.5
trnT-GGU-accD_p4	trnT-GGU-accD	4	TGGCGTTACTCTACCACTGA	CTTGCAATGACCGCTCCTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.127180	59603	62115	2441-2663	0.230769	53.3
trnT-GGU-accD_p5	trnT-GGU-accD	5	GAACCGATGACTTACGCCTT	AGCCCCTCACGTATACTTGT	20	20	50.0	50.0	58.0	58.1	0.0	9.0	0.143989	59603	62115	2369-2591	0.230769	53.2
accD_p1	accD	1	CCGATGACGATGACGAGTAC	AATGCGACCCAACAGGAATT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.096723	62116	64299	2914-2914	0.076923	42.3
accD_p2	accD	2	CCGATGACGATGACGAGTAC	ATGCGACCCAACAGGAATTT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.096723	62116	64299	2913-2913	0.076923	42.3
accD_p3	accD	3	CCGATGACGATGACGAGTAC	TTAGAATGCGACCCAACAGG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.128029	62116	64299	2918-2918	0.076923	42.1
accD_p4	accD	4	TCCGATGACGATGACGAGTA	AATGCGACCCAACAGGAATT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.132613	62116	64299	2915-2915	0.076923	42.0
accD_p5	accD	5	TCCGATGACGATGACGAGTA	ATGCGACCCAACAGGAATTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.132613	62116	64299	2914-2914	0.076923	42.0
psaI-pafII_p1	psaI-pafII	1	ATTTCCGGCAATTGCAATGG	AAAAAGGATAAGGGCCCAGC	20	20	45.0	50.0	58.0	58.1	30.3	27.6	0.160988	65091	65542	588-601	1.000000	82.9
psaI-pafII_p2	psaI-pafII	2	ATTTCCGGCAATTGCAATGG	GGGCCCAGCAGAAATTACTT	20	20	45.0	50.0	58.0	58.1	30.3	19.9	0.174371	65091	65542	577-590	1.000000	82.9
psaI-pafII_p3	psaI-pafII	3	ATTTCCGGCAATTGCAATGG	AAGGGCCCAGCAGAAATTAC	20	20	45.0	50.0	58.0	58.1	30.3	27.6	0.174371	65091	65542	579-592	1.000000	82.8
psaI-pafII_p4	psaI-pafII	4	TAGGCCTAGTATTTCCGGCA	AAAAAGGATAAGGGCCCAGC	20	20	50.0	50.0	57.9	58.1	8.9	27.6	0.225083	65091	65542	598-611	1.000000	82.3
psaI-pafII_p5	psaI-pafII	5	GGCAATTGCAATGGCTTCTT	AAAAAGGATAAGGGCCCAGC	20	20	45.0	50.0	57.9	58.1	32.4	27.6	0.237522	65091	65542	582-595	1.000000	82.3
clpP1_p1	clpP1	1	TCTAAACGAGTCGCGCATAC	GCAATGGGGGTTGATACCAT	20	20	50.0	50.0	58.2	57.9	13.6	0.0	0.267760	75959	78069	2580-2787	1.000000	89.6
clpP1_p2	clpP1	2	CGTTCTTGACAGAGTGGGAG	GCAATGGGGGTTGATACCAT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.158299	75959	78069	2867-2990	0.923077	85.0
clpP1_p3	clpP1	3	CTTGACAGAGTGGGAGCTTC	GCAATGGGGGTTGATACCAT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.178463	75959	78069	2863-2986	0.923077	84.9
clpP1_p4	clpP1	4	GTTCTTGACAGAGTGGGAGC	GCAATGGGGGTTGATACCAT	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.284541	75959	78069	2866-2989	0.923077	84.0
clpP1_p5	clpP1	5	TAGCTGACCCTCTTAGTCCG	GCAATGGGGGTTGATACCAT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.140842	75959	78069	2885-3000	0.846154	82.0
rpl22-rps19_p1	rpl22-rps19	1	GCTTTGTCAGCCGATAGAGA	AGCGATACTAGATCTCGGCG	20	20	50.0	55.0	57.4	58.9	0.0	0.0	1.505960	89663	89726	154-572	0.846154	69.8
rpl22-rps19_p2	rpl22-rps19	2	GCTTTGTCAGCCGATAGAGA	GCGATACTAGATCTCGGCGT	20	20	50.0	55.0	57.4	59.2	0.0	6.8	1.788716	89663	89726	153-571	0.846154	67.5
rpl22-rps19_p3	rpl22-rps19	3	GCTTTGTCAGCCGATAGAGA	CGATACTAGATCTCGGCGTT	20	20	50.0	50.0	57.4	56.7	0.0	6.8	1.841349	89663	89726	152-570	0.846154	67.1
rpl22-rps19_p4	rpl22-rps19	4	GCTTTGTCAGCCGATAGAGA	AAGCGATACTAGATCTCGGC	20	20	50.0	50.0	57.4	56.7	0.0	0.0	1.871983	89663	89726	155-573	0.846154	66.8
rpl22-rps19_p5	rpl22-rps19	5	GCTTTGTCAGCCGATAGAGAT	AGCGATACTAGATCTCGGCG	21	20	47.6	55.0	57.9	58.9	0.0	0.0	2.024545	89663	89726	154-572	0.846154	65.6
ycf1_p1	ycf1	1	TGAACGGACCATATCGGAGA	GACAATTTGGAATGCTGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.075845	116261	116380	740-1289	1.000000	91.4
ycf1_p2	ycf1	2	TGAACGGACCATATCGGAGA	CTTGCCCATATGGAGTGGTC	20	20	50.0	55.0	57.9	58.0	0.0	24.0	0.088637	116261	116380	879-1533	1.000000	91.3
ycf1_p3	ycf1	3	TGAACGGACCATATCGGAGA	ACGCCTTTGAGCACGTATAG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.138939	116261	116380	575-1124	1.000000	90.9
ycf1_p4	ycf1	4	TGAACGGACCATATCGGAGA	CGCATGTGCTTGAAACAGTT	20	20	50.0	45.0	57.9	57.9	0.0	3.2	0.185521	116261	116380	611-1160	1.000000	90.5
ycf1_p5	ycf1	5	TGAACGGACCATATCGGAGA	TGCTATCCCGATTCTCTCGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.078849	116261	116380	974-1502	0.307692	57.0
ycf1_p1	ycf1	1	GACAATTTGGAATGCTGGCC	CGCTCAAGAAACGCCAAATT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.064812	139320	139439	333-807	1.000000	91.5
ycf1_p2	ycf1	2	GACAATTTGGAATGCTGGCC	TGAACGGACCATATCGGAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.075845	139320	139439	740-1289	1.000000	91.4
ycf1_p3	ycf1	3	CTTGCCCATATGGAGTGGTC	CGCTCAAGAAACGCCAAATT	20	20	55.0	45.0	58.0	57.9	24.0	0.0	0.077605	139320	139439	451-1051	1.000000	91.4
ycf1_p4	ycf1	4	TGCTATCCCGATTCTCTCGT	CGCTCAAGAAACGCCAAATT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.067817	139320	139439	552-1020	0.307692	57.0
ycf1_p5	ycf1	5	TGCTATCCCGATTCTCTCGT	TGAACGGACCATATCGGAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.078849	139320	139439	974-1502	0.307692	57.0