Hotspot

ndhF

SSC · 112518-114764 · 2247 bp

Back to Ligusticum

Pi 0.0125

MarkerSeek score 53.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 129
pi: 0.0125
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTGAGGCTCTAGATACGGGA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.0	58.3	2847-2964 bp	0.916667	71.0
ndhF_p2	AAATTGGAAGGGCCTCTCTT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	56.7	58.3	2492-2609 bp	0.916667	69.3
ndhF_p3	AATTGGAAGGGCCTCTCTTT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	56.7	58.3	2491-2608 bp	0.916667	69.3
ndhF_p4	TGAGGCTCTAGATACGGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	56.7	58.3	2846-2963 bp	0.916667	69.1
ndhF_p5	CGAGTTTAAATTGGAAGGGCC	GGGGGTAGGATCCGGTAAAT	47.6	55.0	57.5	58.3	2499-2616 bp	0.916667	67.7