Hotspot

ccsA-ndhD

SSC · 118059-118281 · 223 bp

Back to Ligusticum

Pi 0.0541

MarkerSeek score 73.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 223
variable_sites: 32
pi: 0.0541
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGCGTCTATGGGCTTTCTTA	CAGTTGACAAGGTCGAGGTT	50.0	50.0	57.4	57.8	367-380 bp	1.0	81.9
ccsA-ndhD_p2	GGCGTCTATGGGCTTTCTTA	GTTGACAAGGTCGAGGTTCT	50.0	50.0	57.4	57.5	365-378 bp	1.0	79.7
ccsA-ndhD_p3	GGCGTCTATGGGCTTTCTTA	AGTTGACAAGGTCGAGGTTC	50.0	50.0	57.4	57.5	366-379 bp	1.0	79.6
ccsA-ndhD_p4	GGCGTCTATGGGCTTTCTTA	TCAGTTGACAAGGTCGAGGT	50.0	50.0	57.4	58.6	368-381 bp	1.0	79.1
ccsA-ndhD_p5	GGCGTCTATGGGCTTTCTTA	ATCAGTTGACAAGGTCGAGG	50.0	50.0	57.4	57.2	369-382 bp	1.0	77.8