Hotspot

rpl32-trnL-UAG

SSC · 115975-116907 · 933 bp

Back to Ligusticum

Pi 0.0329
MarkerSeek score 71.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
933
variable_sites
117
pi
0.0329
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1011-1077 bp 1.0 90.6
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 1012-1078 bp 1.0 90.6
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA TCGGATTGGAGAGAGGGTAC 50.0 55.0 58.2 57.9 1559-1625 bp 0.833333 80.6
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CGGATTGGAGAGAGGGTACT 50.0 55.0 58.2 57.9 1558-1624 bp 0.833333 80.6
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA AACCACTCGGATTGGAGAGA 50.0 50.0 58.2 58.1 1611-1631 bp 0.416667 55.7