Hotspot

ndhF-rpl32

SSC · 114765-115812 · 1048 bp

Back to Ligusticum

Pi 0.0255
MarkerSeek score 73.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1048
variable_sites
105
pi
0.0255
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGACGGAAAAAGAAG ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 1616-1989 bp 1.0 91.7
ndhF-rpl32_p2 CCGTGTGAACCAAAACCCTA AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 2023-2396 bp 1.0 91.2
ndhF-rpl32_p3 CCGTGTGAACCAAAACCCTA ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 2022-2395 bp 1.0 91.2
ndhF-rpl32_p4 AAGCGGGTCGATCAAGTAAC AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 1795-2168 bp 0.916667 87.9
ndhF-rpl32_p5 AAGCGGGTCGATCAAGTAAC ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 1794-2167 bp 0.916667 87.9