Hotspot

petA-psbJ

LSC · 64988-65958 · 971 bp

Back to Ligusticum

Pi 0.0405

MarkerSeek score 89.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 111
pi: 0.0405
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 89.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	1127-1204 bp	1.0	82.3
petA-psbJ_p2	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	1128-1205 bp	1.0	81.6
petA-psbJ_p3	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	1132-1209 bp	1.0	80.7
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1114-1191 bp	0.916667	78.6
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1115-1192 bp	0.916667	77.8