Hotspot

psaI-ycf4

LSC · 61435-61845 · 411 bp

Back to Ligusticum

Pi 0.0201

MarkerSeek score 73.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 34
pi: 0.0201
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATGGCAATGGCTTCTTT	TTTTCGCGACCCCGTTATAG	45.0	50.0	57.9	58.1	504-542 bp	1.0	86.9
psaI-ycf4_p2	AGTATTTCCGGCAATGGCAA	TTTTCGCGACCCCGTTATAG	45.0	50.0	58.2	58.1	514-552 bp	1.0	86.5
psaI-ycf4_p3	GCAATGGCAATGGCTTCTTT	GGATAATGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	532-570 bp	0.916667	82.0
psaI-ycf4_p4	GCAATGGCAATGGCTTCTTT	AAAGGATAATGGCCCAGCAG	45.0	50.0	57.9	57.9	535-573 bp	0.916667	81.9
psaI-ycf4_p5	AGTATTTCCGGCAATGGCAA	GGATAATGGCCCAGCAGAAA	45.0	50.0	58.2	57.9	542-580 bp	0.916667	81.6