Hotspot

trnS-GCU-trnG-UCC

LSC · 9334-9886 · 553 bp

Back to Ligusticum

Pi 0.0172

MarkerSeek score 69.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 41
pi: 0.0172
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	GGGGTTACTCGAACGAATCA	45.0	50.0	58.0	57.3	614-650 bp	0.916667	79.3
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	GGGGTTACTCGAACGAATCA	50.0	50.0	58.2	57.3	663-699 bp	0.916667	78.1
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	GGGGTTACTCGAACGAATCA	50.0	50.0	57.6	57.3	600-636 bp	0.916667	76.3
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	GGGTTACTCGAACGAATCACA	45.0	47.6	58.0	58.0	613-649 bp	0.916667	76.2
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GGGGTTACTCGAACGAATCA	55.0	50.0	58.5	57.3	595-631 bp	0.916667	75.5