Hotspot

psbK-psbI

LSC · 8709-8994 · 286 bp

Back to Ligusticum

Pi 0.0314

MarkerSeek score 75.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 31
pi: 0.0314
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	400-533 bp	1.0	88.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	401-534 bp	1.0	88.8
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	50.0	50.0	57.9	58.3	402-535 bp	1.0	87.0
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.3	58.1	397-530 bp	1.0	86.8
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.3	58.1	398-531 bp	1.0	86.8