Hotspot

rps16-trnQ-UUG

LSC · 6863-8100 · 1238 bp

Back to Ligusticum

Pi 0.0226
MarkerSeek score 72.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1238
variable_sites
103
pi
0.0226
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 627-1549 bp 1.0 86.0
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 628-1550 bp 1.0 86.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 638-1560 bp 1.0 85.1
rps16-trnQ-UUG_p4 TGCTTTCTACCACAGCGTTT TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.0 57.3 609-1531 bp 1.0 85.0
rps16-trnQ-UUG_p5 TTGCTTTCTACCACAGCGTT TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.0 57.3 610-1532 bp 1.0 85.0