Hotspot

psbA-trnK-UUU

LSC · 2268-2467 · 200 bp

Back to Ligusticum

Pi 0.0529

MarkerSeek score 76.80

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 33
pi: 0.0529
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	308-339 bp	1.0	86.5
psbA-trnK-UUU_p2	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	309-340 bp	1.0	86.0
psbA-trnK-UUU_p3	TTTTCGGTGCTGGTTATCCA	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.4	57.8	299-330 bp	1.0	84.0
psbA-trnK-UUU_p4	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.8	58.6	319-350 bp	1.0	83.4
psbA-trnK-UUU_p5	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	58.6	57.8	277-308 bp	0.916667	80.7