Hotspot

trnH-GUG-psbA

LSC · 1020-1205 · 186 bp

Back to Ligusticum

Pi 0.0759

MarkerSeek score 74.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 46
pi: 0.0759
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	228-288 bp	1.0	90.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	242-302 bp	1.0	90.4
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	241-301 bp	1.0	90.4
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	243-303 bp	1.0	90.4
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.5	57.9	257-317 bp	1.0	87.6