Hotspot

ndhB

Genome · 144302-146516 · 2215 bp

Back to Ligusticopsis

Pi 0.148
MarkerSeek score 64.30
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
Genome
length_bp
2215
variable_sites
904
pi
0.148
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.88
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 GTTGCGGAAGAACGGACTAT AAGCCCAAAGAGGTGAATGG 50.0 50.0 58.0 58.1 2736-2874 bp 0.214286 51.9
ndhB_p2 GTTGCGGAAGAACGGACTAT ATATAGGCCCGAGTTGGACA 50.0 50.0 58.0 57.9 2855-2993 bp 0.214286 51.5
ndhB_p3 GTTGCGGAAGAACGGACTAT TATAGGCCCGAGTTGGACAT 50.0 50.0 58.0 57.9 2854-2992 bp 0.214286 51.5
ndhB_p4 TAGCCGGAAAGTTATGTGGC AAGCCCAAAGAGGTGAATGG 50.0 50.0 58.0 58.1 2766 bp 0.142857 44.9
ndhB_p5 TAGCCGGAAAGTTATGTGGC TATAGGCCCGAGTTGGACAT 50.0 50.0 58.0 57.9 2884 bp 0.142857 44.7
ndhB_p1 AAGCCCAAAGAGGTGAATGG GTTGCGGAAGAACGGACTAT 50.0 50.0 58.1 58.0 2736-2874 bp 0.214286 51.9
ndhB_p2 ATATAGGCCCGAGTTGGACA GTTGCGGAAGAACGGACTAT 50.0 50.0 57.9 58.0 2855-2993 bp 0.214286 51.5
ndhB_p3 TATAGGCCCGAGTTGGACAT GTTGCGGAAGAACGGACTAT 50.0 50.0 57.9 58.0 2854-2992 bp 0.214286 51.5
ndhB_p4 AAGCCCAAAGAGGTGAATGG TAGCCGGAAAGTTATGTGGC 50.0 50.0 58.1 58.0 2766 bp 0.142857 44.9
ndhB_p5 TATAGGCCCGAGTTGGACAT TAGCCGGAAAGTTATGTGGC 50.0 50.0 57.9 58.0 2884 bp 0.142857 44.7