Hotspot

rps7-trnL-CAA

Genome · 143163-143647 · 485 bp

Back to Ligusticopsis

Pi 0.0578

MarkerSeek score 63.70

Primer available yes

Label rps7-trnL-CAA

Feature Metadata

label_name: rps7-trnL-CAA
feature_type: igs
region: Genome
length_bp: 485
variable_sites: 62
pi: 0.0578
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-trnL-CAA_p1	ATGGCAGAGGCAAATAGAGC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	592-593 bp	0.214286	48.6
rps7-trnL-CAA_p2	ATGGCAGAGGCAAATAGAGC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	594-595 bp	0.214286	48.6
rps7-trnL-CAA_p3	TTGGTTATTAGCGGCATCCC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	716-717 bp	0.214286	48.5
rps7-trnL-CAA_p4	TTGGTTATTAGCGGCATCCC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	718-719 bp	0.214286	48.5
rps7-trnL-CAA_p5	TCCGAATTAGTGGATGCTGC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	658-659 bp	0.214286	48.4